Purpose

Prepare a 50-mL pre-culture of CB2A cells from glycerol stock to scale up for making electrocompetent cells.

Materials & Methods

Materials & Equipment

• 1x CB2A glycerol stock cryovial
• 1x sterile 250-mL flask
• Sterile P20/200 tips
• Shaker in 30°C incubation room

Procedure

https://www.embl.org/groups/protein-expression-purification/protocols/preparation-of-electrocompetent-e-coli-cells/

Results

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Summary

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Hand off

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